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Statistical test Data were subjected to analysis of variance (ANOVA) and Tukey's honestly significant difference (HSD) multiple comparisons were conducted using ProStat software Version 5.5(Poly Software International Inc., Pearl River, NY)

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One of the most affected pathways was glucose metabolism, which includes metabolites such as: D-glucose, lactic acid, citric acid, and mevalonate metabolites, showing significant alterations in G93A-SOD1 compared to control cells