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Recombinant His-tagged AtGrxS15, AtGrxC1 and roGFP2 were also expressed and purified according to established protocols 34,36

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Reducing glutathione production in lymphoma cells, either through pharmacological inhibition of glutamate cysteine ligase (GCL), the enzyme catalyzing the rate-limiting step in glutathione biosynthesis, or via knockdown of GCLC , the GCL catalytic subunit, sharply decreased cell growth in 3D gels and xenografts

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