After centrifugation and thorough removal of the supernatant, the pellets were incubated for 1 h in 60 l staining solution (1% BSA and 0.5% Tween-20 in PBS) containing 3 l anti-BrdUFITC monoclonal antibody (BioLegend, 364104, clone 3D4)
Protein synthesis and regulation
10.1016/j.ajpath.2014.03.005[Epub ahead of print]
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The standardized spray formulation supports investigations into peptide stability, formulation science, intracellular signaling, and intranasal delivery systems while maintaining rigorous analytical quality standards for Research Use Only applications