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Maintaining high levels of glutathione can help to repair free radical damage caused by pollution, stress, and infection
In order to investigate if the substrate binding protein of Gsi (GsiB) transfers GSH to Opp or vice versa (OppA to Gsi), we generated GSH-deficient cells lacking either OppA and GsiC or GsiB and OppC and expressed Grx1-roGFP2 in the resulting gshA gsiB oppC and gshA oppA gsiC triple mutants (see Figure 7 A for a schematic overview)
Reduces expensive trial-and-error experimental screening
Important safety considerations include: it is not recommended during pregnancy or breastfeeding
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