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The hAMY 3 R cells were cultured in growth medium consisting of DMEM (Gibco, #31966-021) supplemented with 10% v/v FBS (Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122), 0.5 mg/mL Geneticin G418 (Gibco, #10131-027), 0.4 mg/mL Hygromycin (Invitrogen, #10687010) and 250 nM Methotrexate (Sigma, A6770)

Longer uninterrupted dosing is not recommended because receptor sensitivity can diminish without cycling

If measuring outcomes like transepidermal water loss, elasticity, or biopsy markers, consistent timing eliminates this confounding variable

They provide important support for mechanistic studies and potential therapeutic strategies for muscle-related diseases

Compounding safety-risk information that includes injectable GHK-Cu

Davies Harpreet S