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The melting curves of amplified products were generated to ensure the specificity of assays at the end of each PCR

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AAV9 virus production, purification, and injection Human embryonic kidney (HEK) 293FT cells were transfected with an AAV9-sgRNA vector 102 , an AAV9 serotype plasmid (pAAV2/9n, 112865, Addgene) and an AAV helper plasmid (pDF6, 112867, Addgene) at a ratio of 1:1:2

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Key structural features worth noting: 15 amino acids (short