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Second, 50 L of the reacted solution, 180 L of AuNPs (10.5 nM), 180 L of BrittonRobinson (BR) buffer (0.04 M H 3 PO 4 , 0.04 M HAc, 0.04 M H 3 BO 3 , pH 3.6), and 90 L of H 2 O were orderly added into a 1.5-mL Eppendorf tube, and the solution was allowed to react for 15 min at room temperature (ca