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For further mitochondrial fractionation experiments, protease protection assays were conducted by incubating intact mitochondria with 0.5 g/mL of proteinase K (K) for 10 min, either alone or following a 5 min pretreatment with 0.2% Triton X-100, and then subjected to western blotting

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Our data showed that pretreatment with a GLP-1R agonist alleviated the expression of ER stress-related proteins, such as PDI, GRP78, CHOP, and caspase-12, which increase in response to ER stress and are considered to be mediators of apoptotic pathways 45,46 , which were partial reversed by LY294002

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