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Bradfield, University of Wisconsin), NDI1 LSL (N

At each sampling point, cellular Fe 2+ was oxidised to Fe 3+ by addition of potassium dichromate (40 mM final concentration) to prevent further peroxide bond activation, followed by protein precipitation with acetonitrile (containing 150 ng/mL of diazepam as the internal standard) as previously described 28

After affinity purification, we will also monitor the purity of the isolated protein using techniques such as SDS-PAGE

The cDNA was then fourfold diluted and used as a template in quantitative PCR with IQ SYBR Green Supermix (Bio-Rad) on a CFX96 instrument (Bio-Rad) using a 3-step protocol

Glutathione supports this process by protecting mitochondria from damage and ensuring efficient energy production

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