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It binds copper(II) with high affinity (association constant in the range of 10 to the power of 16 per molar, placing it among the tighter biological copper chelators)

Expert Review of Anti-infective Therapy 4 :601618

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Biofilm growth of Escherichia coli is subject to cAMP-dependent and cAMP-independent inhibition

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As mentioned earlier, higher doses yield quicker results but may also increase the risk of side effects