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Ann Rheum Dis (2007) 66(5):62832

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For transfection of Lenti-X 293T, cells were seeded in the 6-well plate on the first day and transfected on the second day when the cells reached approximately 70%90% confluent, as described previously ( Confocal fluorescence microscopy analysis Lenti-X 293T cells were seeded on cell slides in 24 well-plate on the first day and transfected on the next day when cells reached 30%50% confluence

Cell-culture medium is not a benign environment in this respect: it commonly contains ascorbate or other reductants