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These trace amounts of deprotection base have minimal effect on the subsequent coupling reaction and are normally tolerated in wash-based SPPS with the understanding that additional washes will have diminishing returns on the quality of the peptide produced and create unnecessary waste
It means your weight should fluctuate no more than 5-10 pounds from your surgery weight to maintain optimal results
Hoffmeyer, Genve (Orthopdie)
Neurosci Biobehav Rev 172:106119
So thats week 2
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