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One significant mechanism involves the production of immunosuppressive factors, with MDSCs secreting high levels of arginase-1 (Arg-1) [11], inducible nitric oxide synthase (iNOS) [12], and reactive oxygen species (ROS) [13], all of which play key roles in dampening T cell activity

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The blood was obtained from the heart and the serum was isolated by allowing the blood to coagulate, followed by centrifugation at 1600 g for 10 min, ensuring the separation of serum from other blood components for subsequent evaluations