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Cells were transfected at 80% confluency using Lipofectamine 2000 (Thermofisher) Briefly, 2 g pcDNA3.2- aptf2::V5 was diluted in 200 l Opti-MEM (Life Technologies) and 8 l Lipofectamine was diluted in 200 l Opti-MEM

The GLP-1 friendly label isnt regulated like other food markers, such as heart healthy or organic, both of which have strict guidelines to be used

No GHS pictograms required

Therefore, it is urgent to validate the therapeutic effect of GLP-1R agonists with large-scale studies and well-designed randomized, controlled trials

The structured dosing limits (8 doses/month, no more than 1/day) reflect the rigorous risk-benefit analysis that only regulatory evaluation can provide