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[51] Diagnosis [edit] According to one review: "At present, no 'gold standard' test exists for the diagnosis of vitamin B 12 deficiency and as a consequence the diagnosis requires consideration of both the clinical state of the patient and the results of investigations." [52] The vitamin deficiency is typically suspected when a routine complete blood count shows anemia with an elevated mean corpuscular volume (MCV)

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Vitamin C is the required coenzyme for two groups of enzymes that catalyze the crosslinking of collagen fibers lysyl hydroxylases and prolyl hydroxylases