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2.2.6 Cortisol For cortisol analyses serum was gained by centrifugation of blood (15 min, 15C, 1950 g, Varifuge 3.0, Heraeus, Hanau, Germany) after incubation for 30 min at room temperature and 30 min at 30C and then stored at -80C until measurement

Let refrigerated medication warm up

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6 : High amounts of surfactants and especially cationic nanoparticles are known to bear high membrane disruption potential