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They will also set an upper limit on the total weekly dose (for example, some experts recommend not exceeding ~300 mg per week in subcutaneous dosing without further evaluation) to minimize any risk of adverse effects

The cells were then treated in triplicate with 2012, 2020 (negative control), and Gambogic Acid (positive control) at titrating concentrations ranging from 100 M to 200 nM and allowed to incubate for 45 min in a 37 C incubator with 5% CO 2

: -20 C

Never freeze reconstituted solution as this destroys the peptide structure

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