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Sigma-Aldrich, Madrid, Spain) and cultured in RPMI 1640 medium supplemented with 10% heat-inactivated FBS, penicillin, streptomycin, L-glutamine, and 50 U/mL of human recombinant interleukin 2 (IL2) [49]

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However, the combination increases the total medication burden and cumulative side-effect risk, which warrants careful medical supervision and baseline health assessment

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Patients with a personal or family history of medullary thyroid carcinoma or multiple endocrine neoplasia type 2 should not use these medications