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discard if the solution becomes cloudy, discolored, or shows particulates

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There should be no bubbles if there are, gently tap the syringe to move them to the top and push them out before proceeding

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One day after growth hormone was added to the tendon fibroblasts treated with BPC 157, the cells were washed with PBS followed by the addition of 1 mL DMEM containing 0.05 mg/mL MTT,, and incubation for 1 hour