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The vector for protein production, pJB-GST-HTS-HS-GFP, was created by first cloning a 7 amino acid spacer fragment containing an XhoI site into pET-15b (Novagen) using sticky ligation and BamHI/XhoI sites

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The elevated concentrations of nicotinamide, the precursor to NAD + , further supports the imbalance in redox state and energy metabolism of the barren habitat sea urchin population in this study 67,68,69