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The cleared lysate was applied to either a pre-equilibrated 5 ml MBPTrap or HisTrap column (GE Healthcare Biosciences) connected to an kta_primeplus device at a flow rate of 1 ml/min

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Salivary cortisol determined by enzyme immunoassay is preferable to serum total cortisol for assessment of dynamic hypothalamic-pituitary-adrenal axis activity

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